Composite

Part:BBa_K3287003:Design

Designed by: Maria Ancin   Group: iGEM19_UPNAvarra_Spain   (2019-10-08)


Hg_Purple


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 462
    Illegal NheI site found at 485
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we do not have any special consideration


Source

We used the MerR activator, which regulates the expression of the Mer operon from Shigella flexneri R100 plasmid Tn21. This part was used for the first time by iGEM team Peking 2010 and then improved by Bielefeld-CeBiTec in iGEM 2015. It activates transcription in response to metal ions (Brown et al., 2003). The specific promoter PmerT was also design by Bielefeld-CeBiTec in 2015. We placed the tsPurple protein behind the PmerT promoter. This chromoprotein was design by the Uppsala-Sweden team in iGEM 2011, exhibiting strong color when expressed.

References